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The first three fragments were, respectively subcloned into pRS425 (leu2 auxotrophic marker) digested by Xho I and Hin dIII, resulting in the multi-copy plasmids pVTC2-GLDH, pALO1-VTC2, and pALO1-GLDH

b-c , Analysis of intestinal distribution of FcR1 expressing cells ( b ) and GDF15 expressing cells ( c ) from control and allergic sensitized WT or IgE KO mice (n = 4 WT control, 5 WT allergic, 4 IgE KO allergic per group)

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Once inside the cell, the -glutamyl-amino acid can be metabolized to release the amino acid and 5-oxoproline, which can then be converted into glutamate to be used in the synthesis of GSH