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They were stained with PE-anti-his-tag antibody (1:50 dilution) and washed

Remarkably, at this higher dose (240 g three times a day), the mean reduction in body weight with pramlintide treatment over 16 weeks was approximately twice that previously observed over a similar time-frame in insulin-treated subjects with type 2 diabetes who were treated with lower pramlintide doses (120 g)

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