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distributed by LGC Standards GmbH, Wesel, Germany) were routinely cultivated in Dulbeccos modified Eagles medium (DMEM)/F-12 (11330057, Thermo Fisher Scientific), supplemented with 10 % FBS and penicillinstreptomycin in a humidified incubator at 37 C with 5% CO 2

When inserting the cigarette, gently press and twist it onto the pinhead

[54] In July 1949, at a fancy-dress ball at the US ambassador's residence, Margaret performed the can-can on stage, accompanied by Douglas and 10 other costumed girls