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DNA cleavage by agarose gel electrophoresis Cleavage reactions were prepared to a final volume of 20 L in 100 L Eppendorf tubes and contained 400 ng supercoiled pUC19 DNA, 1 mM Na-L-ascorbate (where indicated) and 25 mM NaCl in 80 mM HEPES buffer (pH = 7.4)