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Homocysteine causes vascular endothelial dysfunction by disrupting endoplasmic reticulum redox homeostasis

doi: 10.1038/nrm.2016.149 3 FuchsYStellerH

EMSA EMSAs were performed by mixing increasing amounts of wt or mutated BRD4 ET (with or without ASXL1 peptide at a 1:1 molar ratio) with 10 nM/lane of 147 bp 601 DNA in 2025 mM Tris pH 7.5, 150 mM NaCl and 5 mM DTT in a 10 L reaction volume