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Consistent with this, our preclinical work uncovered that TMAO primed renal fibroblasts for conversion to myofibroblasts, the primary collagen-producing cells in the kidney 46 , and contributed to renal fibrosis, a hallmark of kidney damage irrespective of the underlying cause that significantly contributes to eGFR decline 46

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In this case, incorporating computational and statistical methods based on either simple correlations or factorization approaches permits localization scoring, as recently demonstrated for individual organelles (Youn et al., 2018