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The chemical selectivity of Mollugin toward phenol thiols over cysteine showed that carefully considering subtle chemical differences between the war head group and its intended biological target is vital to design both selective and sensitive chemical probes

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Serial applications of oligomycin (ATP synthase inhibitor, 1 M), FCCP (protonophore, 0.5 M), and rotenone and antimycin A (respiratory complex I and III inhibitor, respectively, 0.5 M) over time enabled calculation of various parameters of mitochondrial respiration in both cell lines