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6th edition, January 2020 OptiPrep Application Sheet C21 Dendritic cells from tissues by flotation through a low-density barrier OptiPrep is a 60% (w/v) solution of iodixanol in water, density = 1.32 g/ml The OptiPrep Reference List RC05 Dendritic cells from blood and tissues compares all of the current methodologies and provides a full list of all the published papers reporting the use of OptiPrep: to access return to the initial list of Folders and select Reference Lists To access other Application Sheets referred to in the text return to the Cell Index

Reaction mix with cell lysate and 1 mM ATP, but no F6P, was monitored as blank control