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The sinusoids have a discontinuous, fenestrated endothelial cell lining

Oxidative damage can impair the transcription of the most important genes and lead to the occurrence of mutations

Farhat Z, Browne RW, Bonner MR, Tian L, Deng F, Swanson M, et al

Pellets were thawed on ice and lysed in 2X lysis buffer (100 mM TrisHCl pH 7.5, 300 mM NaCl, 2 mM EDTA, 2% (v/v) IGEPAL CA-630, 1% sodium deoxycholate, 0.5 mM TCEP (Tris(2-carboxyethyl)phosphine hydrochloride) with EDTA-free Protease Inhibitor Cocktail (Sigma-Aldrich) for 20 min on ice