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let dry Draw the calculated dose into a fresh, sterile syringe Remove air bubbles by flicking the syringe and gently pushing out Pinch a fold of skin at the injection site Insert needle at 45 and inject slowly over 510 seconds Withdraw needle, apply gentle pressure with swab (do not rub) Dispose of syringe in sharps container never reuse needles Site Rotation Is Critical: Repeating GHK-Cu injection in the same spot can cause localized irritation or fat tissue changes over time

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[DOI] [PMC free article] [PubMed] [Google Scholar] 120.Karachalias N., Babaei-Jadidi R., Kupich C., Ahmed N., Thornalley P.J