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Cell viability was determined by mixing an aliquot of the suspension 1:2 with trypan blue and counting viable cells using a hemocytometer

After permeabilization and blocking, primary antibody was added and incubated for 1 h at room temperature

Many immunomodulatory factors are found in the tegument, the multi-layered outer surface of the parasite which is capable of quick self-renewal after damage, a process mediated by a population of somatic stem cells with a propensity to differentiate into tegument associated cells (147).The factors within the tegument have been extensively examined (148, 149)

LeeDXuIMChiuDKLeiboldJTseAPBaoMHet al