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Chronic infusion of MSO into rat hippocampus increases glutamate content, specifically in astrocytes, by almost 50%, but has remarkably little effect on glutamate in synaptic endings (Perez et al., Glutamine can travel between gap-coupled astrocytes, and the distance it reaches increases during brain activation (Cruz et al., direct its transport from astrocytes to neurons, but it now appears well established that glutamine release occurs via the amino acid transporter SN1

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4ad, genomic DNA was extracted according to Qiamp Fast DNA Stool extraction kit (Qiagen) and the V3V4 region of the bacterial 16S rRNA gene was amplified with the NEBNext Q5 Hot Start Hifi PCR Master Mix (NEB) using a dual-index strategy