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Analysis of the reaction between LA/DHLA and RNS LA (Cayman Chemical) or DHLA (Sigma-Aldrich) was incubated with the NO donors, GSNO, DPTA NONOate and PAPA NONOate (Cayman Chemical) at room temperature in 20 mM sodium phosphate reaction buffer for 1 or 3 h, as indicated in figure legends

Both phases are accompanied by the secretion of pro-inflammatory cytokines (such as IL-6 and TNF- ), activating neuroglial inflammatory responses and further expanding the damage

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The patients mother had type 2 diabetes mellitus (T2DM) for 10 years, diagnosed at age 35, currently on insulin therapy