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Plasma was isolated and diluted 1:10 in 50% glycerol/PBS solution before stored at 20 C until analysis by ELISA

The saikosaponin family exerts its antidepressant effects through various pathways: promoting ubiquitination of NLRP3 inflammasome, inhibiting NF-B pathway, upregulating the expression of anti-ferroptotic proteins (GPX4, Nrf2, and SLC7A11) and attenuating lipid peroxidation