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Description

Your own cells do the work

2.5 l of this cell suspension was mixed with 7.5 l of lysis buffer [200 mM TrisHCl (pH 7.5), 50 mM EDTA, 0.5% (w/v) SDS] directly on a slide and incubated for 5 min followed by tilting the slides to 30 causing the drops to run down by gravity