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Evaluation of malondialdehyde level, total oxidant/antioxidant status and oxidative stress index in colorectal cancer patients

Barcodes were added at the 5 terminus of the forward primer to allow for sample multiplexing during sequencing 55

They were transfected for 3 h in Opti-MEM I (Invitrogen corp., carlsbad, CA, USA), and then the medium was changed to RPMI 1640 with 5% fetal bovine serum and they were incubated for further 33 h

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